Murine model usefulness for Actinobacillus pleuropneumoniae Virulence sudies

Authors

  • José Ignacio Rodríguez Barbosa
  • César B. Gutiérrez Martín
  • Elías Fernando Rodríguez Ferri
  • José Antonio Vázquez Boland
  • R. I. Tascón Cabrero

DOI:

https://doi.org/10.18002/analesdeveterinaria.v38.7860

Keywords:

Actinobacillus pleuropneumoniae, DL50, murine model, LDso, virulence, iron

Abstract

The usefulness of murine model for Actinobacillus pleuropneumoniae virulence studies was established by means of reference strains and field isolates LD5o determinations. One log differences allowed us to cluster strains in two groups. High virulence strains were those belonging to serotypes 1, 5, 9, 10, and 11, which produce ApxI hemolysin. The second group displayed a lower mortality and included strains of serotypes 2, 3, 4, 6, 7, and 8, which do not express ApxI. The major role of ApxI in A. pleuropneumoniae pathogenicity for mice was evidenced by these results. This animal model allowed us to establish differences between full-virulent strains and non-hemolytic mutants. Nevertheless, mice are unable to reproduce A. pleuropneumoniae natural infection and they seem to be only a good model for acute disease. Iron pool modification by iron dextran and Desferin° inoculation did not significantly improve the murine infection.

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Published

1994-01-02

Issue

Section

Research papers