A new method for determining enzyme kinetic constants

Authors

  • Roberto Martín Sarmiento Universidad de León
  • Justino Burgos González Universidad de León

DOI:

https://doi.org/10.18002/analesdeveterinaria.v25.9145

Abstract

A new procedure is described for calculating kinetic parameters of reactions which obey Michaelis-Menten and derived equations. The performance of this method at several substrate concentration ranges was compared with that of the plots of  l/v  versus l/s, s/v, versus s and v versus v/s and with the procedures of WILKINSON and EISENTHAL & CORNISH-BOWDEN by using simulated rate data containing errors of constant absolute magnitude, constant relative magnitude and intermediate, with and without outliers in each case. The method proved to be excelent in accuracy and precision in all the explored situations.

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Published

2025-05-30

Issue

Section

Research papers